| DATE | HOME | TIME/SCORE | AWAY |
12/3/10 | Hamburger SV | 3 - 1 | Anderlecht |
19/3/10 | Anderlecht | 03:05 | Hamburger SV |
| aggr. | Anderlecht | 3 - 1 | Hamburger SV |
12/3/10 | Rubin Kazan | 1 - 1 | Vfl Wolfsburg |
19/3/10 | Vfl Wolfsburg | 03:05 | Rubin Kazan |
| aggr. | Vfl Wolfsburg | 1 - 1 | Rubin Kazan |
12/3/10 | Atlético Madrid | 0 - 0 | Sporting Lisbon |
19/3/10 | Sporting Lisbon | 03:05 | Atlético Madrid |
| aggr. | Sporting Lisbon | 0 - 0 | Atlético Madrid |
12/3/10 | Lille | 1 - 0 | Liverpool |
19/3/10 | Liverpool | 03:05 | Lille |
| aggr. | Liverpool | 1 - 0 | Lille |
12/3/10 | Panathinaikos | 1 - 3 | Standard Liège |
19/3/10 | Standard Liège | 01:00 | Panathinaikos |
| aggr. | Standard Liège | 1 - 3 | Panathinaikos |
12/3/10 | Valencia | 1 - 1 | Werder Bremen |
19/3/10 | Werder Bremen | 01:00 | Valencia |
| aggr. | Werder Bremen | 1 - 1 | Valencia |
12/3/10 | Benfica | 1 - 1 | Olympique Marseille |
19/3/10 | Olympique Marseille | 01:00 | Benfica |
| aggr. | Olympique Marseille | 1 - 1 | Benfica |
12/3/10 | Juventus | 3 - 1 | Fulham |
19/3/10 | Fulham | 01:00 | Juventus |
| aggr. | Fulham | 3 - 1 | Juventus |
